Characterization of the ATPase Activity of CasDinG

Authors

Christian Cahoon, Utah State UniversityFollow

Files

Description

The battle between bacteria and phage has been ongoing for eons. This battle has generated the evolutionary pressure necessary for the development of microbial immune systems. Characterization of these systems has led to the discovery of molecular tools such CRISPR-Cas systems. This system uses a genetic memory of past viral infections coupled with associated proteins to form ribonucleoprotein complexes which seek out and destroy foreign genetic elements. These systems have been repurposed by scientists to create powerful gene editing tools such as Cas9. With such powerful molecular tools being discovered, we have pursued the characterization of a relatively unknown system, the Type IV-A CRISPR Cas system. Previous work has shown that the Type IV-A CRISPR-associated proteins form a ribonucleoprotein complex, and that an ancillary gene casdinG is required for immune function. The research in this presentation shows the characterization of the ATPase activity of CasDinG, an XPD family helicase. We show that ATP hydrolysis is substantially increased in the presence of nucleic acid.

Publication Date

12-9-2021

City

Logan, UT

Keywords

CRISPR, ATP, hydrolysis, microbial immune systems

Disciplines

Biochemistry

Recommended Citation

Cahoon, Christian, "Characterization of the ATPase Activity of CasDinG" (2021). Fall Student Research Symposium 2021. 12.
https://digitalcommons.usu.edu/fsrs2021/12