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Description

CRISPR-Cas systems are adaptive prokaryotic immune systems that enable host cells to defend against attack from foreign nucleic acids such as phage infections or plasmids. CRISPR-Cas systems are diverse and encompass 2 classes, 6 types, and 33 subtypes. The Type IV-A CRISPR-Cas system from Pseudomonas aeruginosa strain 83 is composed of five different genes (csf1, csf2, csf3, cas6, and dinG). Type IV-A systems are poorly understood, and currently there is little research detailing their biological and biochemical mechanism of immunity. CasDinG, an ancillary protein within the Type IV-A system, is required for an immune response in vivo. However, the role of CasDinG at the molecular level is still not understood. In this research, I present recent advances detailing the biochemical function of CasDinG. To elucidate these data, various biochemical methods were used including: bioinformatic analyses, helicase assays, and fluorescence anisotropy.

Publication Date

12-9-2021

City

Logan, UT

Keywords

CRISPR, nucleic acids, proteins, helicase assays

Disciplines

Physical Sciences and Mathematics

Determining the Biochemical Mechanisms and Binding Activities of a Type IV-A CRISPR-Cas DinG Helicase

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