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Description

Increasing incidences of drought forced farmers to use the secondary, degraded water for irrigation. These water sources are rich in salt concentrations. This project has started with the hopes of finding bacteria from the rhizosphere of a native to Utah plant, Ceanothus velutinus, that helps the plant survive the saltier conditions of Utah. The rhizosphere, a layer of soil attached to the roots of a plant, contains microorganisms that may contribute to the plants' abiotic and biotic stress resistance. These microorganisms are known as Plant Growth Promoting Rhizobacteria (PGPR). The roots and rhizosphere samples were collected from Tony Grove in Logan, Utah. The samples were collected from three different elevations (1920m, 1950m, and 2289m). The rhizosphere bacteria were isolated at 37°C and 28°C on 2,4,6,8 and 10% NaCl concentration in Nutrient Agar media. Twenty colonies from the first screen at 37°C were identified by 16SrRNA sequencing and characterized for nitrogen fixation, phosphate solubilization, and siderophore production. In the second screen at 28°C three bacteria from the 6% and 8% salt concentrations were isolated. Once these bacteria will be identified and characterized, they will be tested on the plants like Arabidopsis and Medicago to see their aid in contributing to plant health at varying levels of soil salinity.

Publication Date

12-9-2021

City

Logan, UT

Keywords

drought, salt concentration, bacteria, irrigation

Disciplines

Plant Sciences

Halotolerant Rhizosphere Bacteria: Isolation of Rhizosphere Bacteria From Native Utah Plant <em>Ceanothus velutinus</em>

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