Expressing and Purifying Type IV CRISPR Accessory Proteins

Authors

Alivia Jolley, Utah State UniversityFollow

Files

Description

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR-associated) adaptive immune systems defend bacteria and archaea against phages, plasmids, and other mobile genetic elements. Each Type IV CRISPR system contains a subtype-specific gene and is hypothesized to be essential for function. The Type IV-A system encodes ATP-dependent 5'-3' DNA helicase called CasDinG, while the Type IV-B systems encode a putative pyrophosphatase named CasCysH. Here we investigate the function of the type IV CRISPR systems by expressing, purifying, and characterizing the accessory proteins from the type IV systems. This project is a first step towards understanding the structure and mechanism of type IV systems and the possibility of repurposing type IV systems in future applications.

Publication Date

12-5-2022

City

Logan, UT

Keywords

accessory proteins, CRISPR, genes, type IV systems

Disciplines

Life Sciences

Recommended Citation

Jolley, Alivia, "Expressing and Purifying Type IV CRISPR Accessory Proteins" (2022). Fall Student Research Symposium 2022. 47.
https://digitalcommons.usu.edu/fsrs2022/47