Date of Award
5-2004
Degree Type
Thesis
Degree Name
Departmental Honors
Department
Chemistry and Biochemistry
Abstract
Acetone carboxylase, an inducible multimeric enzyme found in several bacteria, catalyzes the carboxylation of acetone to produce acetoacetate. This is an important reaction for the bacteria because, upon addition of coenzyme A, the acetoacetate can be converted to acetyl-CoA, a common metabolic intermediate. Azotobacter vinelandii, one of the bacteria that produces acetone carboxylase, has two different genes encoding this enzyme. We knocked out the first of these by disrupting it with a kanamycin cassette to help us understand the function of the second gene. Knocking out the gene will also make possible the use of site-directed mutagenesis to further study the enzyme.
Recommended Citation
Gardner, Jessica, "Genetic Analysis of Acetone Carboxylase in Azotobacter vinelandii" (2004). Undergraduate Honors Capstone Projects. 783.
https://digitalcommons.usu.edu/honors/783
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Faculty Mentor
Scott Ensign
Departmental Honors Advisor
Steve Scheiner