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Dynamics of Stomatal Patches for a Single Surface of Xanthium Strumarium L. Leaves Observed with Fluorescence and Thermal Images

Document Type

Article

Journal/Book Title/Conference

Plant, Cell, & Environment

Volume

28

Issue

5

Publisher

Published by Wiley-Blackwell in Plant, Cell, & Environment

Publication Date

5-2005

First Page

633

Last Page

641

Abstract

Fluorescence and thermal imaging were used to examine the dynamics of stomatal patches for a single surface of Xanthium strumarium L. leaves following a decrease in ambient humidity. Patches were not observed in all experiments, and in many experiments the patches were short-lived. In some experiments, however, patches persisted for many hours and showed complex temporal and spatial patterns. Rapidly sampled fluorescence images showed that the measurable variations of these patches were sufficiently slow to be captured by fluorescence images taken at 3-min intervals using a saturating flash of light. Stomatal patchiness with saturating flashes of light was not demonstrably different from that without saturating flashes of light, suggesting that the regular flashes of light did not directly cause the phenomenon. Comparison of simultaneous fluorescence and thermal images showed that the fluorescence patterns were largely the result of stomatal conductance patterns, and both thermal and fluorescence images showed patches of stomatal conductance that propagated coherently across the leaf surface. These nondispersing patches often crossed a given region of the leaf repeatedly at regular intervals, resulting in oscillations in stomatal conductance for that region. The existence of these coherently propagating structures has implications for the mechanisms that cause patchy stomatal behaviour as well as for the physiological ramifications of this phenomenon.

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https://doi.org/10.1111/j.1365-3040.2005.01309.x

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