Photoacoustic Studies of Carboxymyoglobin
Pulsed-laser photoacoustics offers a means for measuring the Fe-CO bond strength in carboxymyoglobin, as well as the dynamics of the protein following cleavage of the Fe-CO bond. Two groups have studied the problem in the past, with conflicting results [Leung et al., Chem. Phys. Letts. 141, 220 (1987); Westrick et al., Biochemistry 26, 8313 (1987)]. Preliminary results from our laboratory support a positve value for the enthalpy of formation of the Fe:CO geminate pair, formed within 10 nsec of photolysis of carboxymyoglobin. Data will be presented on the Fe-CO bond energy, and the volumetric and enthalpic changes which occur in myoglobin following Fe-CO bond cleavage.
Photoacoustic studies of carboxymyoglobin. S. L. Larson and J. Rudzki Small. Biophysical J. 57, 229a (1990).