Title

Glycolate Oxidase Activity Assay in Plants

Document Type

Article

Journal/Book Title/Conference

Bio-protocol

Volume

2

Issue

20

Publisher

Bio-protocol

Publication Date

10-20-2012

Abstract

Glycolate oxidase is located in the peroxisome and is involved in the photorespiratory cycle which recovers some of the carbon loss during photosynthesis. Glycolate oxidase converts glycolate to glyxoylate with the concomitant production of H2O2. In this assay, the H2O2 generated, in the presence of HRP, oxidizes O-dianisidine into a colored O-dianisidine radical cation that can be quantified spectrophotometrically. The amount of color produces is directly proportional to the glycolate oxidase activity.

Comments

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