Measurement of NADPH Oxidase Activity in Plants

Document Type

Article

Journal/Book Title/Conference

Bio-protocol

Volume

2

Issue

20

Publisher

Bio-protocol

Publication Date

10-20-2012

Abstract

NADPH oxidase is a membrane-bound enxyme that generates (O2-) by transferring electrons from NADPH to molecular oxygen O2. O2- is spontaneously dismasted to the more stable form H2O2. Both O2- and H2O2 are forms of reactive oxygen species (ROS), which are involved in regulation of many cellular activities such as transcription, intracellular signaling, and host defense. The NADPH oxidase - dependent generation of O2- in total membrane fraction of plant tissue has been determined by the reduction of the tetrazolium salt XTT by O2-. In the presense of O2-, XTT generates a soluble yellow formazan that can be quantified spectrophotometrically.

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