Measurement of NADPH Oxidase Activity in Plants
NADPH oxidase is a membrane-bound enxyme that generates (O2-) by transferring electrons from NADPH to molecular oxygen O2. O2- is spontaneously dismasted to the more stable form H2O2. Both O2- and H2O2 are forms of reactive oxygen species (ROS), which are involved in regulation of many cellular activities such as transcription, intracellular signaling, and host defense. The NADPH oxidase - dependent generation of O2- in total membrane fraction of plant tissue has been determined by the reduction of the tetrazolium salt XTT by O2-. In the presense of O2-, XTT generates a soluble yellow formazan that can be quantified spectrophotometrically.
Kaundal, A., Rojas, C. M. and Mysore, K. S. (2012). Measurement of NADPH Oxidase Activity in Plants. Bio-protocol 2(20): e278. DOI: 10.21769/BioProtoc.278.