Title of Oral/Poster Presentation

In-vitro fluorescence imaging, surface-enhanced Raman spectroscopy and photothermal therapy of human lung adenocarcinoma epithelial cells by CaMoO4:Eu@Au hybrid nanoparticles

Class

Article

Department

Biological and Irrigation Engineering

Faculty Mentor

Anhong Zhou

Presentation Type

Oral Presentation

Abstract

Multifunctional Eu3+-doped CaMoO4@Au-nanorods (GNR) core/shell nanoparticles (NPs) were synthesized for fluorescence imaging, SERS detection and PTT applications. Anti-epidermal growth factor receptor (EGFR) antibodies were conjugated with the synthesized NPs to enhance the specificity because EGFR, as a biomarker of cancer, was overexpressed on human lung adenocarcinoma epithelial cells (A549). The red fluorescence of the synthesized NPs coms from the Europium ion (Eu3+). The GNR component serves both as a SERS-active and PTT substrates. By conjugating with a Raman reporter molecule, 4-mercaptobenzoic acid (MBA), it generates SERS signals. Meantime, heat can be rapidly generated by 808 nm near-infrared (NIR) laser irradiation of the prepared NPs. Fluorescence microscopy exhibited that these particles largely located around cellular cytoplasm. Meantime, Raman mapping confirmed the distribution of these NPs by SERS characteristic peak selection. In addition, these NPs effectively suppressed A549 cells viability upon 808 nm laser irradiation. Thus, this study shows the potential of CaMoO4:Eu@GNR NPs with fluorescence imaging, SERS detection and PTT functionalities.

Start Date

4-9-2015 11:00 AM

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Apr 9th, 11:00 AM

In-vitro fluorescence imaging, surface-enhanced Raman spectroscopy and photothermal therapy of human lung adenocarcinoma epithelial cells by CaMoO4:Eu@Au hybrid nanoparticles

Multifunctional Eu3+-doped CaMoO4@Au-nanorods (GNR) core/shell nanoparticles (NPs) were synthesized for fluorescence imaging, SERS detection and PTT applications. Anti-epidermal growth factor receptor (EGFR) antibodies were conjugated with the synthesized NPs to enhance the specificity because EGFR, as a biomarker of cancer, was overexpressed on human lung adenocarcinoma epithelial cells (A549). The red fluorescence of the synthesized NPs coms from the Europium ion (Eu3+). The GNR component serves both as a SERS-active and PTT substrates. By conjugating with a Raman reporter molecule, 4-mercaptobenzoic acid (MBA), it generates SERS signals. Meantime, heat can be rapidly generated by 808 nm near-infrared (NIR) laser irradiation of the prepared NPs. Fluorescence microscopy exhibited that these particles largely located around cellular cytoplasm. Meantime, Raman mapping confirmed the distribution of these NPs by SERS characteristic peak selection. In addition, these NPs effectively suppressed A549 cells viability upon 808 nm laser irradiation. Thus, this study shows the potential of CaMoO4:Eu@GNR NPs with fluorescence imaging, SERS detection and PTT functionalities.