Microfluidic Chip for Single Cell Analysis of Tumor Cells Interaction with Anti-Cancer Drug Doxorubicin by AFM and Raman Microspectroscopy
Class
Article
Department
Biological and Irrigation Engineering
Faculty Mentor
Anhong Zhou
Presentation Type
Oral Presentation
Abstract
The advantages of Raman spectroscopy include high efficient, high specific and label free, and therefore, the method is also eligible to live cells. In this study, a important technical innovation is reported here towards Raman cell identification. A microfluidic chip made of MgF2 is introduced for Raman cell natural spectral fingerprint collection. The cancer cells were cultured in device for a period of 3 days to validate the biocompatibility of the microfluidic system. In addition, Both AFM and Raman spectroscopy were applied to detect the responses of A549, 231 and 231-B cells to DOX exposure (24h) at the single cell level. Principal component analysis (PCA) and atomic force microscope (AFM) results basically agree with our previously published results tested by traditional method. The Microfluidic cell culture platform is straightforward, reusable and it has not been reported in the literature. This microfluidic device system provide a new platform for Raman cancer cell research and clinic cancer diagnostic.
Start Date
4-9-2015 11:00 AM
Microfluidic Chip for Single Cell Analysis of Tumor Cells Interaction with Anti-Cancer Drug Doxorubicin by AFM and Raman Microspectroscopy
The advantages of Raman spectroscopy include high efficient, high specific and label free, and therefore, the method is also eligible to live cells. In this study, a important technical innovation is reported here towards Raman cell identification. A microfluidic chip made of MgF2 is introduced for Raman cell natural spectral fingerprint collection. The cancer cells were cultured in device for a period of 3 days to validate the biocompatibility of the microfluidic system. In addition, Both AFM and Raman spectroscopy were applied to detect the responses of A549, 231 and 231-B cells to DOX exposure (24h) at the single cell level. Principal component analysis (PCA) and atomic force microscope (AFM) results basically agree with our previously published results tested by traditional method. The Microfluidic cell culture platform is straightforward, reusable and it has not been reported in the literature. This microfluidic device system provide a new platform for Raman cancer cell research and clinic cancer diagnostic.