Class
Article
College
College of Science
Faculty Mentor
Ryan Jackson
Presentation Type
Oral Presentation
Abstract
The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats - CRISPR ASsociated) system is an adaptive bacterial immune system. Upon infection by a foreign nucleic acid, such as phage, the bacterium's CRISPR-Cas system takes a chunk of the foreign nucleic acid and inserts it into the bacterium's own genome at the CRISPR locus. The CRISPR locus is then transcribed and processed into CRISPR RNAs (crRNAs) which now contain a sequence complementary to the foreign nucleic acid inserted into the CRISPR. The crRNAs are used by the CRISPR-Cas system to recognize, through complementary base pairing with the crRNA, and destroy foreign nucleic acids which have previously infected the bacteria. There are many types of CRISPR-Cas systems. In Type I and III CRISPR systems, the CRISPR is processed into crRNAs by the Cas6 endoribonuclease. Bioinformatic studies classify Type IV CRISPR systems as Class 1 systems, like Type I and III, and have identified a putative Cas6 endoribonuclease that is associated with Type IV systems. This talk discusses the first biochemical characterization and structure of the Type IV Cas6 endoribonuclease.
Location
Room 101
Start Date
4-12-2018 9:00 AM
End Date
4-12-2018 10:15 AM
CRISPR RNA processing by a Type IV-A Cas6 endoribonuclease
Room 101
The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats - CRISPR ASsociated) system is an adaptive bacterial immune system. Upon infection by a foreign nucleic acid, such as phage, the bacterium's CRISPR-Cas system takes a chunk of the foreign nucleic acid and inserts it into the bacterium's own genome at the CRISPR locus. The CRISPR locus is then transcribed and processed into CRISPR RNAs (crRNAs) which now contain a sequence complementary to the foreign nucleic acid inserted into the CRISPR. The crRNAs are used by the CRISPR-Cas system to recognize, through complementary base pairing with the crRNA, and destroy foreign nucleic acids which have previously infected the bacteria. There are many types of CRISPR-Cas systems. In Type I and III CRISPR systems, the CRISPR is processed into crRNAs by the Cas6 endoribonuclease. Bioinformatic studies classify Type IV CRISPR systems as Class 1 systems, like Type I and III, and have identified a putative Cas6 endoribonuclease that is associated with Type IV systems. This talk discusses the first biochemical characterization and structure of the Type IV Cas6 endoribonuclease.