Title of Oral/Poster Presentation

CRISPR RNA processing by a Type IV-A Cas6 endoribonuclease

Class

Article

College

College of Science

Faculty Mentor

Ryan Jackson

Presentation Type

Oral Presentation

Abstract

The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats - CRISPR ASsociated) system is an adaptive bacterial immune system. Upon infection by a foreign nucleic acid, such as phage, the bacterium's CRISPR-Cas system takes a chunk of the foreign nucleic acid and inserts it into the bacterium's own genome at the CRISPR locus. The CRISPR locus is then transcribed and processed into CRISPR RNAs (crRNAs) which now contain a sequence complementary to the foreign nucleic acid inserted into the CRISPR. The crRNAs are used by the CRISPR-Cas system to recognize, through complementary base pairing with the crRNA, and destroy foreign nucleic acids which have previously infected the bacteria. There are many types of CRISPR-Cas systems. In Type I and III CRISPR systems, the CRISPR is processed into crRNAs by the Cas6 endoribonuclease. Bioinformatic studies classify Type IV CRISPR systems as Class 1 systems, like Type I and III, and have identified a putative Cas6 endoribonuclease that is associated with Type IV systems. This talk discusses the first biochemical characterization and structure of the Type IV Cas6 endoribonuclease.

Location

Room 101

Start Date

4-12-2018 9:00 AM

End Date

4-12-2018 10:15 AM

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Apr 12th, 9:00 AM Apr 12th, 10:15 AM

CRISPR RNA processing by a Type IV-A Cas6 endoribonuclease

Room 101

The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats - CRISPR ASsociated) system is an adaptive bacterial immune system. Upon infection by a foreign nucleic acid, such as phage, the bacterium's CRISPR-Cas system takes a chunk of the foreign nucleic acid and inserts it into the bacterium's own genome at the CRISPR locus. The CRISPR locus is then transcribed and processed into CRISPR RNAs (crRNAs) which now contain a sequence complementary to the foreign nucleic acid inserted into the CRISPR. The crRNAs are used by the CRISPR-Cas system to recognize, through complementary base pairing with the crRNA, and destroy foreign nucleic acids which have previously infected the bacteria. There are many types of CRISPR-Cas systems. In Type I and III CRISPR systems, the CRISPR is processed into crRNAs by the Cas6 endoribonuclease. Bioinformatic studies classify Type IV CRISPR systems as Class 1 systems, like Type I and III, and have identified a putative Cas6 endoribonuclease that is associated with Type IV systems. This talk discusses the first biochemical characterization and structure of the Type IV Cas6 endoribonuclease.