Class
Article
College
College of Agriculture and Applied Sciences
Faculty Mentor
Craig Day
Presentation Type
Poster Presentation
Abstract
My project compares several different methods of freezing human cell lines for storage to see which method best preserves the cells. Cell storage is an important but often overlooked part of any lab that does in vitro testing. Freezing cells allows cell lines to be used long after they are harvested, and allows laboratories to use cells at much lower passage numbers than if they had been continuously grown since harvest. It is important to store the cells properly because poor storage methods can seriously harm or kill valuable cell lines. To test different cell freezing strategies, I will harvest stocks of Huh7 cells, a continuous cell line derived from a human liver. I will freeze the cells with different methods. Then, I will then thaw the cells and allow them to grow overnight and then count the cells to see which freezing method preserved the cells’ viability the best.
Location
The South Atrium
Start Date
4-12-2018 12:00 PM
End Date
4-12-2018 1:15 PM
Economically Preserving Mammalian Cell Cultures at the Institute for Antiviral Research
The South Atrium
My project compares several different methods of freezing human cell lines for storage to see which method best preserves the cells. Cell storage is an important but often overlooked part of any lab that does in vitro testing. Freezing cells allows cell lines to be used long after they are harvested, and allows laboratories to use cells at much lower passage numbers than if they had been continuously grown since harvest. It is important to store the cells properly because poor storage methods can seriously harm or kill valuable cell lines. To test different cell freezing strategies, I will harvest stocks of Huh7 cells, a continuous cell line derived from a human liver. I will freeze the cells with different methods. Then, I will then thaw the cells and allow them to grow overnight and then count the cells to see which freezing method preserved the cells’ viability the best.