Class
Article
College
College of Science
Department
Biology Department
Faculty Mentor
Abby Benninghoff
Presentation Type
Poster Presentation
Abstract
Colorectal cancer is currently the second leading cause of cancer-related deaths in the United States. The risk of colon cancer increases in individuals who suffer from colitis, which is inflammation of the colon lining. Colitis is common in patients with inflammatory bowel disease (IBD). The development of colorectal cancer is complex and can be associated with dysbiosis of the gut microbiota, which has been shown to be affected by diet. Disruption of the gut microbiome can lead to the overgrowth of pathogenic bacteria which can contribute to disease. Pathogenic bacteria and other bacterial byproducts that manage to cross the epithelial barrier, trigger the immune system. Small, nonstructural proteins called cytokines are critical to immune response and may have either pro-inflammatory or anti-inflammatory effects. Two cytokines involved in inflammatory response associated with the development of colitis-associated colorectal cancer include IL-6 and IL-17. IL-6 is released by immune cells in response to some other inflammatory cytokines as part of the pro-inflammation signaling pathway that is often active in many chronic disease conditions associated with inflammation. Similarly, IL-17 is also a pro-inflammatory cytokine produced T helper 17 cells that play an important role in maintaining the mucosal barrier of the intestine and help clear pathogens. The goal of this study is to determine the blood concentrations of certain cytokines in mice fed either healthy diet (AIN93G) or a Western style diet (TWD) prior to, during, and after colitis, as well as during tumorigenesis. Concentration of the two cytokines in blood samples will be determined using a commercial enzyme-linked immunosorbent assay kit. Data collection and analyses are still ongoing. We expect blood levels of IL-6 and IL-17 to increase in animals fed a TWD diet indicating higher levels of colitis, and the levels to remain elevated through recovery and tumorigenesis, indicating adverse systemic inflammation.Presentation Time: Thursday, 11 a.m.-12 p.m.
Location
Logan, UT
Start Date
4-11-2021 12:00 AM
Included in
Cytokine Indicators of Inflammation in Mice fed a Western Type Diet
Logan, UT
Colorectal cancer is currently the second leading cause of cancer-related deaths in the United States. The risk of colon cancer increases in individuals who suffer from colitis, which is inflammation of the colon lining. Colitis is common in patients with inflammatory bowel disease (IBD). The development of colorectal cancer is complex and can be associated with dysbiosis of the gut microbiota, which has been shown to be affected by diet. Disruption of the gut microbiome can lead to the overgrowth of pathogenic bacteria which can contribute to disease. Pathogenic bacteria and other bacterial byproducts that manage to cross the epithelial barrier, trigger the immune system. Small, nonstructural proteins called cytokines are critical to immune response and may have either pro-inflammatory or anti-inflammatory effects. Two cytokines involved in inflammatory response associated with the development of colitis-associated colorectal cancer include IL-6 and IL-17. IL-6 is released by immune cells in response to some other inflammatory cytokines as part of the pro-inflammation signaling pathway that is often active in many chronic disease conditions associated with inflammation. Similarly, IL-17 is also a pro-inflammatory cytokine produced T helper 17 cells that play an important role in maintaining the mucosal barrier of the intestine and help clear pathogens. The goal of this study is to determine the blood concentrations of certain cytokines in mice fed either healthy diet (AIN93G) or a Western style diet (TWD) prior to, during, and after colitis, as well as during tumorigenesis. Concentration of the two cytokines in blood samples will be determined using a commercial enzyme-linked immunosorbent assay kit. Data collection and analyses are still ongoing. We expect blood levels of IL-6 and IL-17 to increase in animals fed a TWD diet indicating higher levels of colitis, and the levels to remain elevated through recovery and tumorigenesis, indicating adverse systemic inflammation.Presentation Time: Thursday, 11 a.m.-12 p.m.