Class
Article
College
College of Engineering
Department
Biological Engineering Department
Faculty Mentor
Elizabeth Vargis
Presentation Type
Poster Presentation
Abstract
The rapidly expanding field of scaffolding within tissue engineering has produced plant tissue decellularization techniques to create cellulose scaffolds that replicate complex vasculature. Spinacia oleracea (Spinach) leaves have been the primary focus of decellularization research in creating scaffolding due to their wide availability and highly vascularized structure. To expand on previous research, this experiment has been conducted to compare S. oleracea, Brassica oleracea var. sabellica (kale) and Lactuca sativa (romaine lettuce) in the visualization and density of vasculature. The decellularization process has been adapted from previous literature using the detergent sodium lauryl sulfate to remove cellular matter and residual DNA. The method included various washing and soaking steps in order to clear the plant matter and produce quality scaffolding for cell growth in well plates. Results were determined through the ability of the chosen plants to be fully decellularized and function as simple scaffolding for cell growth. Further research is being conducted to determine biocompatibility of vascular endothelial cells on scaffold surfaces. Presentation Time: Thursday, 2-3 p.m.
Location
Logan, UT
Start Date
4-11-2021 12:00 AM
Included in
Plant Tissue Decellularization for Complex Vascular Scaffold Applications
Logan, UT
The rapidly expanding field of scaffolding within tissue engineering has produced plant tissue decellularization techniques to create cellulose scaffolds that replicate complex vasculature. Spinacia oleracea (Spinach) leaves have been the primary focus of decellularization research in creating scaffolding due to their wide availability and highly vascularized structure. To expand on previous research, this experiment has been conducted to compare S. oleracea, Brassica oleracea var. sabellica (kale) and Lactuca sativa (romaine lettuce) in the visualization and density of vasculature. The decellularization process has been adapted from previous literature using the detergent sodium lauryl sulfate to remove cellular matter and residual DNA. The method included various washing and soaking steps in order to clear the plant matter and produce quality scaffolding for cell growth in well plates. Results were determined through the ability of the chosen plants to be fully decellularized and function as simple scaffolding for cell growth. Further research is being conducted to determine biocompatibility of vascular endothelial cells on scaffold surfaces. Presentation Time: Thursday, 2-3 p.m.