Class
Article
College
Emma Eccles Jones College of Education and Human Services
Department
Kinesiology & Health Sciences
Faculty Mentor
Abby Benninghoff
Presentation Type
Poster Presentation
Abstract
A variety of genetic and environmental factors can affect the composition of the human gut microbiota, including a poor diet. Western diets are typically associated with dysbiosis and other adverse health outcomes that are expressed phenotypically. The primary objective of this study is to determine the contribution of gut microbiota from mice donors who have previously consumed one of two basal diets: 1) the standard AIN93G diet, which is designed to promote rodent health; and 2) the total Western diet (TWD), which promotes inflammation-associated colorectal tumorigenesis. The donors’ microbiota were transferred to a cohort of mice who were fed either the AIN93G diet or the TWD in order to see the impact of the microbiota on inflammation and tumorigenesis. Using a 2x2 factorial design, C57BL/6J male mice were fed the standard AIN93G diet or the total Western diet (TWD) for 16 weeks while receiving fecal microbiota transfer (FMT) from the mice fed either the AIN93G diet or the TWD in a prior study. Prior to fecal transfer, the resident gut microbiome was depleted using an established antibiotic/antifungal oral dosing regimen. The azoxymethane + dextran sodium sulfate model of inflammation-associated colorectal cancer was employed to assess the dynamic response of the gut microbiome to basal diet and FMT treatment prior to, during, and after active colitis and at the study end. Endpoints assessed include body weight gain, body composition, food and energy intake, and organ weights. Preliminary data analyses suggest that total food intake and final body weights of mice were similar regardless of diet or FMT source. Also, our preliminary analysis points to decreased colon length in mice fed a TWD supplemented with TWD-FMT, and indicator of elevated colitis. In conclusion, the source of FMT may contribute to the developing disease state in mice fed a Western style diet. Presentation Time: Wednesday, 12-1 p.m.
Location
Logan, UT
Start Date
4-11-2021 12:00 AM
Included in
Impact of Fecal Microbiota Transfer (FMT) on Phenotype of Mice Fed a Standard Diet and a Western-Style Diet Using a Colitis-Associated Colorectal Cancer Model
Logan, UT
A variety of genetic and environmental factors can affect the composition of the human gut microbiota, including a poor diet. Western diets are typically associated with dysbiosis and other adverse health outcomes that are expressed phenotypically. The primary objective of this study is to determine the contribution of gut microbiota from mice donors who have previously consumed one of two basal diets: 1) the standard AIN93G diet, which is designed to promote rodent health; and 2) the total Western diet (TWD), which promotes inflammation-associated colorectal tumorigenesis. The donors’ microbiota were transferred to a cohort of mice who were fed either the AIN93G diet or the TWD in order to see the impact of the microbiota on inflammation and tumorigenesis. Using a 2x2 factorial design, C57BL/6J male mice were fed the standard AIN93G diet or the total Western diet (TWD) for 16 weeks while receiving fecal microbiota transfer (FMT) from the mice fed either the AIN93G diet or the TWD in a prior study. Prior to fecal transfer, the resident gut microbiome was depleted using an established antibiotic/antifungal oral dosing regimen. The azoxymethane + dextran sodium sulfate model of inflammation-associated colorectal cancer was employed to assess the dynamic response of the gut microbiome to basal diet and FMT treatment prior to, during, and after active colitis and at the study end. Endpoints assessed include body weight gain, body composition, food and energy intake, and organ weights. Preliminary data analyses suggest that total food intake and final body weights of mice were similar regardless of diet or FMT source. Also, our preliminary analysis points to decreased colon length in mice fed a TWD supplemented with TWD-FMT, and indicator of elevated colitis. In conclusion, the source of FMT may contribute to the developing disease state in mice fed a Western style diet. Presentation Time: Wednesday, 12-1 p.m.