Class
Article
College
College of Agriculture and Applied Sciences
Department
Biology Department
Faculty Mentor
Lee Rickords
Presentation Type
Poster Presentation
Abstract
DDX3 is a ubiquitous gene in mammals that codes for a protein in the DEAD-box protein family. This protein is an ATP-dependent RNA helicase, which denotes its involvement in altering the structure of RNA1, functions as a transcriptional regulator1, and is proposed to be involved in the nuclear export of retroviral RNA1. The DDX3 gene is located on the X-chromosome between base pair 34,831,836 and base pair 34,847,409 (15,573 total base pairs). In addition, there is a homologous gene located on the Y-chromosome between base pair 6,194,312 to bp 6,199,142 (4,917 total base pairs). The Y-chromosome homolog, however, contains unique deletion regions, making it a suitable candidate for a PCR-based sex identification study. The purpose of this study was to design a primer pair that has a built in PCR amplification control to identify sex in equine (Equus caballus) blood and tissue samples. The methods of this study were to first align the DDX3X gene with the Y-homolog and create primer pairs to flank two Y-chromosome specific deletions. Amplicons for the X- and Y-chromosome specific genes were 310 base pairs and 371 base pairs, respectively. Second, blood samples were collected from 40 total equine subjects, approximately 28 females and 10 males. Results showed confirmation of the hypothesis that this primer pair can be used to accurately identify separate X- and Y-chromosome specific sequences via a single primer pair in equine forensic samples. This presentation will provide information on the conducted study, as well as suggest possible further research for DNA conservation of the DDX3 gene in humans, canines, and mandrils. Presentation Time: Wednesday, 11 a.m.-12 p.m. Zoom link: https://usu-edu.zoom.us/j/87115208381?pwd=NVZiTjZ4Y1l1QnVwNDMzWmUydE9lZz09
Location
Logan, UT
Start Date
4-11-2021 12:00 AM
Included in
Novel Approach for Sex Identification in Equine Forensic Samples Using PCR Amplification
Logan, UT
DDX3 is a ubiquitous gene in mammals that codes for a protein in the DEAD-box protein family. This protein is an ATP-dependent RNA helicase, which denotes its involvement in altering the structure of RNA1, functions as a transcriptional regulator1, and is proposed to be involved in the nuclear export of retroviral RNA1. The DDX3 gene is located on the X-chromosome between base pair 34,831,836 and base pair 34,847,409 (15,573 total base pairs). In addition, there is a homologous gene located on the Y-chromosome between base pair 6,194,312 to bp 6,199,142 (4,917 total base pairs). The Y-chromosome homolog, however, contains unique deletion regions, making it a suitable candidate for a PCR-based sex identification study. The purpose of this study was to design a primer pair that has a built in PCR amplification control to identify sex in equine (Equus caballus) blood and tissue samples. The methods of this study were to first align the DDX3X gene with the Y-homolog and create primer pairs to flank two Y-chromosome specific deletions. Amplicons for the X- and Y-chromosome specific genes were 310 base pairs and 371 base pairs, respectively. Second, blood samples were collected from 40 total equine subjects, approximately 28 females and 10 males. Results showed confirmation of the hypothesis that this primer pair can be used to accurately identify separate X- and Y-chromosome specific sequences via a single primer pair in equine forensic samples. This presentation will provide information on the conducted study, as well as suggest possible further research for DNA conservation of the DDX3 gene in humans, canines, and mandrils. Presentation Time: Wednesday, 11 a.m.-12 p.m. Zoom link: https://usu-edu.zoom.us/j/87115208381?pwd=NVZiTjZ4Y1l1QnVwNDMzWmUydE9lZz09