Class
Article
College
College of Agriculture and Applied Sciences
Department
Plants, Soils, and Climate Department
Faculty Mentor
Amita Kaundal
Presentation Type
Poster Presentation
Abstract
Climatic change is one of the biggest threats to the ecosystem and biodiversity by enhancing environmental stresses. Environmental stresses such as biotic and abiotic stresses affect plant health and reduce crop production. The rhizosphere microbiome of a plant plays a significant role in a plant's defense against various biotic and abiotic stresses. In this study, we are investigating the microbiome diversity of bulk soil, rhizosphere, and endosphere of Ceanothus velutinus, snowbrush. Ceanothus is an evergreen native plant that is usually found in dry areas and thrives well in harsh conditions. The snowbrush samples were collected from different locations 1920m, 1950m, and 2289m of the Tony Grove area of the Intermountain West region of the United States. The snowbrush plants propagated from cuttings under the greenhouse conditions were treated with the native soil. The rhizosphere and roots samples were collected from treatment and control plants after 3 months of inoculation for microbiome studies. The DNA was isolated from all the samples of native plants and greenhouse plants and sequenced for 16s rRNA for bacteria and ITS for fungi. The NGS data has been analyzed by the QIIME tool to investigate microbial diversity. The results revealed the dominance of Proteobacteria followed by Actinobacteria and Acidobacteria in all the bulk soil; Actinobacteria, Proteobacteria and Gemmatimonadetes in rhizosphere; Proteobacteria, Actinobacteria and Bacteroidetes in endosphere native soil samples; Proteobacteria, Actinobacteria and Verrucomicrobia in the control and treatment samples from the greenhouse. There were a few phyla that were absent in control but present in the treated plants and the native soil like Nitrospirae. The taxonomic classification of the native soil samples revealed the presence of various Plant Growth Promoting Rhizobacteria (PGPR) which were also found in the treated plants in the greenhouse but absent in control. They include Rhodococcus that helps in phosphate solubilization. Dyadobacter that fixes atmospheric nitrogen and Sphingobium that helps in siderophore production. The ITS sequencing analysis of the native soil samples revealed the presence of an Arbuscular Mycorrhizal Fungi (AMF) that helps in glycoprotein production in plants. These microorganisms will further be isolated, characterized for their functions in promoting plant growth and development. Presentation Time: Wednesday, 3-4 p.m.
Location
Logan, UT
Start Date
4-12-2021 12:00 AM
Included in
Climate Commons, Plant Sciences Commons, Soil Science Commons
Comparative Study of the Microbiome of the Native Plant Ceanothus velutinus (Snowbrush) From Different Locations and Greenhouse Studies
Logan, UT
Climatic change is one of the biggest threats to the ecosystem and biodiversity by enhancing environmental stresses. Environmental stresses such as biotic and abiotic stresses affect plant health and reduce crop production. The rhizosphere microbiome of a plant plays a significant role in a plant's defense against various biotic and abiotic stresses. In this study, we are investigating the microbiome diversity of bulk soil, rhizosphere, and endosphere of Ceanothus velutinus, snowbrush. Ceanothus is an evergreen native plant that is usually found in dry areas and thrives well in harsh conditions. The snowbrush samples were collected from different locations 1920m, 1950m, and 2289m of the Tony Grove area of the Intermountain West region of the United States. The snowbrush plants propagated from cuttings under the greenhouse conditions were treated with the native soil. The rhizosphere and roots samples were collected from treatment and control plants after 3 months of inoculation for microbiome studies. The DNA was isolated from all the samples of native plants and greenhouse plants and sequenced for 16s rRNA for bacteria and ITS for fungi. The NGS data has been analyzed by the QIIME tool to investigate microbial diversity. The results revealed the dominance of Proteobacteria followed by Actinobacteria and Acidobacteria in all the bulk soil; Actinobacteria, Proteobacteria and Gemmatimonadetes in rhizosphere; Proteobacteria, Actinobacteria and Bacteroidetes in endosphere native soil samples; Proteobacteria, Actinobacteria and Verrucomicrobia in the control and treatment samples from the greenhouse. There were a few phyla that were absent in control but present in the treated plants and the native soil like Nitrospirae. The taxonomic classification of the native soil samples revealed the presence of various Plant Growth Promoting Rhizobacteria (PGPR) which were also found in the treated plants in the greenhouse but absent in control. They include Rhodococcus that helps in phosphate solubilization. Dyadobacter that fixes atmospheric nitrogen and Sphingobium that helps in siderophore production. The ITS sequencing analysis of the native soil samples revealed the presence of an Arbuscular Mycorrhizal Fungi (AMF) that helps in glycoprotein production in plants. These microorganisms will further be isolated, characterized for their functions in promoting plant growth and development. Presentation Time: Wednesday, 3-4 p.m.