Class

Article

College

College of Agriculture and Applied Sciences

Department

English Department

Faculty Mentor

Amita Kaundal

Presentation Type

Poster Presentation

Abstract

Ceanothus velutinusis an evergreen shrub native to the Intermountain West region of the US, commonly known as snowbrush. The center for water-efficient landscaping (CWEL) recommended this plant for sustainable landscaping as its moderately drought tolerant. The roots of these plants form an actinorhizal symbiotic relationship with Nitrogen fixing actinobacteria Frankia, which initiates root nodules formation. Besides, these plants also harbor Plant growth-promoting bacteria (PGPB) that help plants in nutrient uptake, plant hormone production, protecting the plants from ethylene stress, and production of metabolites, thus playing an essential role in plant growth and development. They can be used as biofertilizers to substitute chemical fertilizers. In this study, we isolated and purified bacteria from the nodules of greenhouse-grown Ceanothus velutinus treated with native soil on four bacterial growth media Nutrient agar, Tryptic soy agar, Yeast mannitol agar, and Frankia basal media. These bacterial isolates are purified and identified by 16SrRNA sequencing. They are then characterized for plant growth-promoting activities such as nitrogen fixation, phosphorous solubilization, and siderophore production.

Location

Logan, UT

Start Date

4-8-2022 12:00 AM

Included in

Life Sciences Commons

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Apr 8th, 12:00 AM

Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria From the Root Nodules of Ceanothus velutinus

Logan, UT

Ceanothus velutinusis an evergreen shrub native to the Intermountain West region of the US, commonly known as snowbrush. The center for water-efficient landscaping (CWEL) recommended this plant for sustainable landscaping as its moderately drought tolerant. The roots of these plants form an actinorhizal symbiotic relationship with Nitrogen fixing actinobacteria Frankia, which initiates root nodules formation. Besides, these plants also harbor Plant growth-promoting bacteria (PGPB) that help plants in nutrient uptake, plant hormone production, protecting the plants from ethylene stress, and production of metabolites, thus playing an essential role in plant growth and development. They can be used as biofertilizers to substitute chemical fertilizers. In this study, we isolated and purified bacteria from the nodules of greenhouse-grown Ceanothus velutinus treated with native soil on four bacterial growth media Nutrient agar, Tryptic soy agar, Yeast mannitol agar, and Frankia basal media. These bacterial isolates are purified and identified by 16SrRNA sequencing. They are then characterized for plant growth-promoting activities such as nitrogen fixation, phosphorous solubilization, and siderophore production.