Class
Article
College
College of Engineering
Department
English Department
Faculty Mentor
David Britt
Presentation Type
Poster Presentation
Abstract
The aim of this project is to design a test to demonstrate the effectiveness of a coating applied to the lumen of peritoneal catheter extensions to reduce biofilm formation. Hemodialysis and peritoneal dialysis (PD) patients currently struggle with infections caused by biofilm growth in the intraluminal tubing. Once established, biofilms are very difficult to get rid of and can cause reoccurring infection issues which can lead to a discontinuation of PD, poorer quality of life, and a greater risk of death. Fresenius Medical Care (FMC) has recognized similar problems with their patients and seeks to reduce infection rates. Fresenius Medical Care has found a coating that could be applied to the lumen of dialysis catheter tubing that is designed to reduce bacterial adhesion and thereby reduce biofilm formation and infection rates in patients. This project focuses on developing a protocol to test the effectiveness of this coating on peritoneal dialysis catheter extension tubing, which connects the patient to the dialysate solution tubing. The catheter extension will be simulated using a small silicone disk, or coupon, and placed into a bacterial solution of known concentration for a set amount of time, and analyzed for biofilm formation and reduction. So far, testing has been done with Pseudomonas chlororaphis O6 (PcO6), a bacterium with robust biofilm formation. Results have been analyzed by plating of colony-forming units, crystal violet staining, and atomic force microscopy.
Location
Logan, UT
Start Date
4-8-2022 12:00 AM
Included in
Assessing Bacterial Adhesion to Metallic Coating on Silicone for Peritoneal Dialysis Catheters
Logan, UT
The aim of this project is to design a test to demonstrate the effectiveness of a coating applied to the lumen of peritoneal catheter extensions to reduce biofilm formation. Hemodialysis and peritoneal dialysis (PD) patients currently struggle with infections caused by biofilm growth in the intraluminal tubing. Once established, biofilms are very difficult to get rid of and can cause reoccurring infection issues which can lead to a discontinuation of PD, poorer quality of life, and a greater risk of death. Fresenius Medical Care (FMC) has recognized similar problems with their patients and seeks to reduce infection rates. Fresenius Medical Care has found a coating that could be applied to the lumen of dialysis catheter tubing that is designed to reduce bacterial adhesion and thereby reduce biofilm formation and infection rates in patients. This project focuses on developing a protocol to test the effectiveness of this coating on peritoneal dialysis catheter extension tubing, which connects the patient to the dialysate solution tubing. The catheter extension will be simulated using a small silicone disk, or coupon, and placed into a bacterial solution of known concentration for a set amount of time, and analyzed for biofilm formation and reduction. So far, testing has been done with Pseudomonas chlororaphis O6 (PcO6), a bacterium with robust biofilm formation. Results have been analyzed by plating of colony-forming units, crystal violet staining, and atomic force microscopy.