Class
Article
College
College of Humanities and Social Sciences
Department
English Department
Faculty Mentor
Amita Kaundal
Presentation Type
Poster Presentation
Abstract
Natural medicines with antimicrobial capabilities often contain secondary metabolites from any number of plants or fungi. These plant-derived compounds can have great medicinal or agricultural value. Artemisia tridentata is said to contain antimicrobial compounds and has been used as a medicinal herb in Native American cultural medicine. Artemisia annua, a Chinese herb, was used to derive artemisinin, a common antimalarial drug and potential anticancer treatment. Very few studies exist to research the identity of any antimicrobial compounds in A. tridentata.This study is to identify any antimicrobial compounds in A. tridentata. We collected, dried, and crushed local samples of three different parts of the plant—twigs, leaves, and flowers. We then created an oil extract using methanol and dissolved it in DMSO. We soaked paper disks in the extracts and tested antimicrobial activity by disk diffusion antimicrobial assay. We tested eachoil extractagainst Agrobacterium tumefaciens, Pseudomonas syringae pv DC3000, P.syringae pv tabaci, Bacillus subtilis, and a cloning strain of E. coli Dh5α. We found that the extract was effective at inhibiting growth of each bacteria, except E. coli. B. subtilis showed the clearest results, with large zones of inhibition similar in size to those surrounding our gentamycin positive control disks. We have also tested the extract against several BSL 2 pathogens, with equal success.Upon positive results, we created and standardized our methodology for the following process. We separated the compounds within the extract via High Performance Liquid Chromatography (HPLC) and are testing each of the fractions individually against B. subtilis through disk diffusion. We then plan to identify the antimicrobial compound within the plant extract through Liquid Chromatography-Mass Spectrometry (LCMS) analysis and comparison with the chemical library. The discovery of any actively antimicrobial compound, whether known or previously undiscovered, will be valuable due to the abundance and availability of A. tridentata in the western United States.
Location
Logan, UT
Start Date
4-8-2022 12:00 AM
Included in
Antimicrobial Activity of Artemisia tridentata
Logan, UT
Natural medicines with antimicrobial capabilities often contain secondary metabolites from any number of plants or fungi. These plant-derived compounds can have great medicinal or agricultural value. Artemisia tridentata is said to contain antimicrobial compounds and has been used as a medicinal herb in Native American cultural medicine. Artemisia annua, a Chinese herb, was used to derive artemisinin, a common antimalarial drug and potential anticancer treatment. Very few studies exist to research the identity of any antimicrobial compounds in A. tridentata.This study is to identify any antimicrobial compounds in A. tridentata. We collected, dried, and crushed local samples of three different parts of the plant—twigs, leaves, and flowers. We then created an oil extract using methanol and dissolved it in DMSO. We soaked paper disks in the extracts and tested antimicrobial activity by disk diffusion antimicrobial assay. We tested eachoil extractagainst Agrobacterium tumefaciens, Pseudomonas syringae pv DC3000, P.syringae pv tabaci, Bacillus subtilis, and a cloning strain of E. coli Dh5α. We found that the extract was effective at inhibiting growth of each bacteria, except E. coli. B. subtilis showed the clearest results, with large zones of inhibition similar in size to those surrounding our gentamycin positive control disks. We have also tested the extract against several BSL 2 pathogens, with equal success.Upon positive results, we created and standardized our methodology for the following process. We separated the compounds within the extract via High Performance Liquid Chromatography (HPLC) and are testing each of the fractions individually against B. subtilis through disk diffusion. We then plan to identify the antimicrobial compound within the plant extract through Liquid Chromatography-Mass Spectrometry (LCMS) analysis and comparison with the chemical library. The discovery of any actively antimicrobial compound, whether known or previously undiscovered, will be valuable due to the abundance and availability of A. tridentata in the western United States.