Class
Article
College
College of Agriculture and Applied Sciences
Department
Plants, Soils, and Climate Department
Faculty Mentor
Youping Sun
Presentation Type
Poster Presentation
Abstract
Phalaenopsis sp., commonly known as moth orchids, is one of the most popular orchids sold as cut flowers or potted plants because of their high ornamental value. Cross pollination of seed parent (Phalaenopsis mannii x Phalaenopsis ‘Yaphong Javaking’) and pollen parent (Phalaenopsis schilleriana) was conducted to obtain hybrids which presumably possess the combined traits and characteristics from the parent plants. Seeds were collected 112 days after hand pollination and sown in five germination media: half-strength Murashige and Skoog Medium (⅟2 MS), Vacin and Went Medium (VW), Orchid Seed Sowing Medium (OSSM), BM-1 Terrestrial Orchid Medium (BM-1), and Knudson C Orchid Medium (KC). After 16 weeks of culture, the highest seed germination was achieved on OSSM (82.1%), followed by BM-1 (63.1%), KC (62.0%), ⅟2 MS (40.8%), and VW (31.8%). Seeds also germinated faster on OSSM compared to other media, with 25.1% seeds showing advanced stage of germination after 16 weeks of culture. Eighteen-week-old seedlings from the OSSM medium were transferred to five Orchid Multiplication Medium (OMM) supplemented with 0.5 or 1 mg/l of NAA (1-Naphthaleneacetic acid) and 1 or 2 mg/l of BA (6-Benzylaminopurine). After 24 weeks of culture, seedlings were taller in OMM medium supplemented with 1 mg/l BA + 1 mg/l NAA or 2 mg/l BA + 1 mg/l NAA compared to those with 1 mg/l BA + 0.5 mg/l NAA. Fresh weight, leaf length, leaf number, leaf width, root length, and root number were similar in all five media. However, seedlings grown in the medium supplemented with 2 mg/l BA and 1 mg/l NAA developed the highest number of shoots per seedling (2.5). Furthermore, the seedlings will undergo acclimatization in a greenhouse to observe different traits from parent plants.
Location
Logan, UT
Start Date
4-12-2023 1:30 PM
End Date
4-12-2023 2:30 PM
Included in
Study on In Vitro Seed Germination and Seedling Development of New Phalaenopsis Hybrid
Logan, UT
Phalaenopsis sp., commonly known as moth orchids, is one of the most popular orchids sold as cut flowers or potted plants because of their high ornamental value. Cross pollination of seed parent (Phalaenopsis mannii x Phalaenopsis ‘Yaphong Javaking’) and pollen parent (Phalaenopsis schilleriana) was conducted to obtain hybrids which presumably possess the combined traits and characteristics from the parent plants. Seeds were collected 112 days after hand pollination and sown in five germination media: half-strength Murashige and Skoog Medium (⅟2 MS), Vacin and Went Medium (VW), Orchid Seed Sowing Medium (OSSM), BM-1 Terrestrial Orchid Medium (BM-1), and Knudson C Orchid Medium (KC). After 16 weeks of culture, the highest seed germination was achieved on OSSM (82.1%), followed by BM-1 (63.1%), KC (62.0%), ⅟2 MS (40.8%), and VW (31.8%). Seeds also germinated faster on OSSM compared to other media, with 25.1% seeds showing advanced stage of germination after 16 weeks of culture. Eighteen-week-old seedlings from the OSSM medium were transferred to five Orchid Multiplication Medium (OMM) supplemented with 0.5 or 1 mg/l of NAA (1-Naphthaleneacetic acid) and 1 or 2 mg/l of BA (6-Benzylaminopurine). After 24 weeks of culture, seedlings were taller in OMM medium supplemented with 1 mg/l BA + 1 mg/l NAA or 2 mg/l BA + 1 mg/l NAA compared to those with 1 mg/l BA + 0.5 mg/l NAA. Fresh weight, leaf length, leaf number, leaf width, root length, and root number were similar in all five media. However, seedlings grown in the medium supplemented with 2 mg/l BA and 1 mg/l NAA developed the highest number of shoots per seedling (2.5). Furthermore, the seedlings will undergo acclimatization in a greenhouse to observe different traits from parent plants.