Class
Article
College
College of Engineering
Department
Biological Engineering Department
Faculty Mentor
Jixun Zhan
Presentation Type
Poster Presentation
Abstract
Natural products as a source of medicine have long been attractive due to the huge structural diversity and promising biological activities such as antimicrobial, anticancer, antitumor, antifungal, antiviral, antiparasitic as well as antioxidant properties. Discovery of novel and modified natural products is the need of this modern era. To expand the chemical diversity in natural products and to increase the production titers of natural products, researchers have come up with various solutions: heterologous expression, cocultivation, fermentation engineering, isolation of new species, and strain engineering/improvement. o-Coumaric acid and p-coumaric acid are phenolic antioxidants found in various plant sources. These also serve as the precursors for the biosynthesis of various natural products. In this study, several Streptomyces strains were screened for the ability to make novel derivatives of p‑coumaric acid and o-coumaric acid. HPLC was used for products analysis, isolation, and purification. NMR and MS were used to elucidate the structures. Among five strains, Streptomyces sp. NRRL B1677 was found to bio-transform the two substrates into various products. One of the products from p‑coumaric acid was identified as caffeic acid. We cloned two putative 3-monooxygenase genes of Streptomyces sp. NRRL B1677 into pET28a and expressed the resulting plasmids (pHS8 and pHS10 ) in E. coli BL21(DE3). Results indicate that only the strain harboring pHS10 can bio-transform p-CA into caffeic acid, but production titer is low. These genes have also been cloned into pRM5 to yield pHS13 and pHS14, respectively, which will be tested for the function in Streptomyces lividans K4. Future work includes the identification of other products and kinetic studies on the monooxygenase from Streptomyces sp. NRRL B1677.
Location
Logan, UT
Start Date
4-11-2023 12:30 PM
End Date
4-11-2023 1:30 PM
Included in
Natural Product Biosynthesis Through Biotechnological and Fermentation Approaches
Logan, UT
Natural products as a source of medicine have long been attractive due to the huge structural diversity and promising biological activities such as antimicrobial, anticancer, antitumor, antifungal, antiviral, antiparasitic as well as antioxidant properties. Discovery of novel and modified natural products is the need of this modern era. To expand the chemical diversity in natural products and to increase the production titers of natural products, researchers have come up with various solutions: heterologous expression, cocultivation, fermentation engineering, isolation of new species, and strain engineering/improvement. o-Coumaric acid and p-coumaric acid are phenolic antioxidants found in various plant sources. These also serve as the precursors for the biosynthesis of various natural products. In this study, several Streptomyces strains were screened for the ability to make novel derivatives of p‑coumaric acid and o-coumaric acid. HPLC was used for products analysis, isolation, and purification. NMR and MS were used to elucidate the structures. Among five strains, Streptomyces sp. NRRL B1677 was found to bio-transform the two substrates into various products. One of the products from p‑coumaric acid was identified as caffeic acid. We cloned two putative 3-monooxygenase genes of Streptomyces sp. NRRL B1677 into pET28a and expressed the resulting plasmids (pHS8 and pHS10 ) in E. coli BL21(DE3). Results indicate that only the strain harboring pHS10 can bio-transform p-CA into caffeic acid, but production titer is low. These genes have also been cloned into pRM5 to yield pHS13 and pHS14, respectively, which will be tested for the function in Streptomyces lividans K4. Future work includes the identification of other products and kinetic studies on the monooxygenase from Streptomyces sp. NRRL B1677.