Presenter Information

Jeremy Case, Utah State University

Class

Article

College

College of Agriculture and Applied Sciences

Department

Animal, Dairy, and Veterinary Sciences Department

Faculty Mentor

Abby Benninghoff

Presentation Type

Poster Presentation

Abstract

The National Cancer Institute reports colorectal cancer as the leading cause of cancer death for individuals under 50 in the United States (American Cancer Society). These rates continue to rise with poor diet, microbiome dysbiosis, and gut inflammation being the primary drivers.

Our lab performs nutrient- intervention studies on DSS/AOM colitis-induced mouse models fed the total Western diet, reflective of the average American’s diet. A poor diet promotes gut microbiome dysbiosis and subsequent colitis, ultimately yielding colon cancer. We seek to understand how the introduction of a bioactive compound may lessen or promote these effects in in vivo mouse models.

The objective of this project was to develop an in vitro experimental model that representatively cultures the gut microbiome, providing deeper insight into direct interactions between bioactive compounds and the gut microbiome. This methodology will provide wider access to this field of research by using common materials found in labs.

Location

Logan, UT

Start Date

4-9-2025 1:30 PM

End Date

4-9-2025 2:20 PM

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Apr 9th, 1:30 PM Apr 9th, 2:20 PM

Establishing Methods for In Vitro Static Culture of Gut Microbiomes for Nutritional Intervention Studies

Logan, UT

The National Cancer Institute reports colorectal cancer as the leading cause of cancer death for individuals under 50 in the United States (American Cancer Society). These rates continue to rise with poor diet, microbiome dysbiosis, and gut inflammation being the primary drivers.

Our lab performs nutrient- intervention studies on DSS/AOM colitis-induced mouse models fed the total Western diet, reflective of the average American’s diet. A poor diet promotes gut microbiome dysbiosis and subsequent colitis, ultimately yielding colon cancer. We seek to understand how the introduction of a bioactive compound may lessen or promote these effects in in vivo mouse models.

The objective of this project was to develop an in vitro experimental model that representatively cultures the gut microbiome, providing deeper insight into direct interactions between bioactive compounds and the gut microbiome. This methodology will provide wider access to this field of research by using common materials found in labs.