Title

Transgenic Animal Production an Animal Biotechnology

Document Type

Article

Journal/Book Title/Conference

Theriogenology

Volume

67

Publication Date

1-1-2007

First Page

127

Last Page

133

DOI

10.1016/j.theriogenology.2006.09.034

Abstract

Considerable progress has been made in methods for production of transgenic livestock; beginning with pronuclear microinjection over 20 years ago. New methods, including the use of viral vectors, sperm-mediated gene transfer and somatic cell cloning, have overcome many of the limitations of pronuclear microinjection. It is now possible to not only readily make simple insertional genetic modifications, but also to accomplish, more complex, homozygous gene targeting and artificial chromosome transfer in livestock.

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