Document Type
Article
Author ORCID Identifier
Renata Blocher https://orcid.org/0009-0006-5233-5357
Ying Liu https://orcid.org/0000-0002-5680-5110
Tayler Patrick https://orcid.org/0000-0001-7989-9634
Irina A. Polejaeva https://orcid.org/0000-0002-0858-5889
Journal/Book Title/Conference
Animals
Volume
14
Issue
12
Publisher
MDPI AG
Publication Date
6-20-2024
Journal Article Version
Version of Record
First Page
1
Last Page
14
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Abstract
Bovine in vitro oocyte maturation (IVM) is an easy way to obtain oocytes for subsequent assisted reproductive techniques but is inefficient compared to in vivo maturation. Supplementation of three cytokines, fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1), or FLI, has increased oocyte maturation and embryo development in multiple species, but studies have not explored the oocyte differences caused by FLI IVM supplementation. This study aimed to assess important nuclear and cytoplasmic maturation events in high-quality oocytes. FLI-supplemented oocytes had a decreased GV (3.0% vs. 13.7%, p < 0.01) and increased telophase I incidence (34.6% vs. 17.6%, p < 0.05) after IVM, increased normal meiotic spindles (68.8% vs. 50.0%, p < 0.001), and an increased nuclear maturation rate (75.1% vs. 66.8%, p < 0.001). Moreover, in metaphase II oocytes, the percentage of FLI-treated oocytes with a diffuse mitochondrial distribution was higher (87.7% vs. 77.5%, p < 0.05) and with a cortical mitochondrial distribution was lower (11.6% vs. 17.4%, p < 0.05). Additionally, FLI-supplemented oocytes had more pattern I cortical granules (21.3% vs. 14.4%, p < 0.05). These data suggest that FLI supplementation in bovine in vitro maturation medium coordinates nuclear and cytoplasmic maturation to produce higher-quality oocytes.
Recommended Citation
Blocher, R.; Liu, Y.; Patrick, T.; Polejaeva, I.A. Cytokine-Supplemented Maturation Medium Enhances Cytoplasmic and Nuclear Maturation in Bovine Oocytes. Animals 2024, 14, 1837. https://doi.org/10.3390/ani14121837
