Differential Hepatic Expression of miRNA in Response to Aflatoxin B1 Challenge in Domestic and Wild Turkeys

Authors

Kade Jorud, University of Minnesota - St Paul
Kristelle M. Mendoza, University of Minnesota - St Paul
Thomas Kono, University of Minnesota - St Paul
Roger A. Coulombe, Utah State UniversityFollow
Kent M. Reed, University of Minnesota - St PaulFollow

Document Type

Article

Author ORCID Identifier

Kade Jorud https://orcid.org/0009-0000-5180-6424

Thomas Kono https://orcid.org/0000-0002-1388-3194

Journal/Book Title/Conference

Toxins

Volume

16

Issue

11

Publisher

MDPI AG

Publication Date

10-22-2024

Journal Article Version

Version of Record

First Page

1

Last Page

14

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.

Abstract

Aflatoxin B1 (AFB₁) is a major foodborne mycotoxin that poses a significant economic risk to poultry due to a greater degree of susceptibility compared to other agricultural species. Domesticated turkeys (Meleagris gallopavo) are especially sensitive to AFB₁; however, wild turkeys (M. g. silvestris) are more resistant. A lack of functional isoforms of hepatic glutathione S-transferases (GSTs), an enzyme that plays a role in the detoxification of aflatoxin, is suspected as the reason for the increased sensitivity. Previous studies comparing the gene expression of domesticated and wild turkeys exposed to AFB₁ identified hepatic genes responding differentially to AFB₁, but could not fully explain the difference in response. The current study examined differences in the expression of microRNAs (miRNAs) in the livers of wild and domesticated turkeys fed dietary AFB₁ (320 μg/kg in feed). Short-read RNA sequencing and expression analysis examined both domesticated and wild turkeys exposed to AFB₁ compared to controls. A total of 25 miRNAs was identified as being significantly differentially expressed (DEM) in pairwise comparisons. The majority of these have mammalian orthologs with known dysregulation in liver disease. The largest number of DEMs occurred between controls, suggesting an underlying difference in liver potential. Sequences of the DEMs were used to identify potential miRNA binding sites in target genes, resulting in an average of 4302 predicted target sites per DEM. These DEMs and gene targets provide hypotheses for future investigations into the role of miRNAs in AFB₁ resistance.

Recommended Citation

Jorud, K.; Mendoza, K.M.; Kono, T.; Coulombe, R.A.; Reed, K.M. Differential Hepatic Expression of miRNA in Response to Aflatoxin B1 Challenge in Domestic and Wild Turkeys. Toxins 2024, 16, 453. https://doi.org/10.3390/toxins16110453