Document Type
Article
Author ORCID Identifier
Katie A. Shira https://orcid.org/0000-0002-2289-440X
Kara J. Thornton https://orcid.org/0000-0002-7506-5293
Gabrielle M. Becker https://orcid.org/0000-0002-1455-6443
Gordon K. Murdoch https://orcid.org/0000-0002-8643-7777
Journal/Book Title/Conference
PLoS One
Volume
19
Issue
7
Publisher
Public Library of Science
Publication Date
7-3-2024
Journal Article Version
Version of Record
First Page
1
Last Page
20
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Abstract
Skeletal muscle growth is an economically important trait in the cattle industry. Secreted muscle-derived proteins, referred to as myokines, have important roles in regulating the growth, metabolism, and health of skeletal muscle in human and biomedical research models. Accumulating evidence supports the importance of myokines in skeletal muscle and whole-body health, though little is known about the potential presence and functional significance of these proteins in cattle. This study evaluates and confirms that secreted proteins acidic and rich in cysteine (SPARC), fibroblast growth factor 21 (FGF-21), myostatin (MSTN), and decorin (DCN) are expressed and SPARC, FGF-21, and DCN are secreted by primary bovine satellite cells from 3- (BSC3; n = 3) and 11- (BSC11; n = 3) month -old commercial angus steers. Cells were cultured and collected at zero, 12, 24, and 48 hours to characterize temporal expression and secretion from undifferentiated and differentiated cells. The expression of SPARC was higher in the undifferentiated (p = 0.04) and differentiated (p = 0.07) BSC11 than BSC3. The same was observed with protein secretion from undifferentiated (p < 0.0001) BSC11 compared to BSC3. Protein secretion of FGF-21 was higher in undifferentiated BSC11 (p < 0.0001) vs. BSC3. DCN expression was higher in differentiated BSC11 (p = 0.006) vs. BSC3. Comparing undifferentiated vs. differentiated BSC, MSTN expression was higher in differentiated BSC3 (p ≤ 0.001) for 0, 12, and 24 hours and in BSC11 (p ≤ 0.03) for 0, 12, 24, and 48 hours. There is also a change over time for SPARC expression (p ≤ 0.03) in undifferentiated and differentiated BSC and protein secretion (p < 0.0001) in undifferentiated BSC, as well as FGF-21 expression (p = 0.007) in differentiated BSC. This study confirms SPARC, FGF-21, and DCN are secreted, and SPARC, FGF-21, MSTN, and DCN are expressed in primary bovine muscle cells with age and temporal differences.
Recommended Citation
Shira KA, Thornton KJ, Murdoch BM, Becker GM, Chibisa GE, Murdoch GK (2024) Expression and secretion of SPARC, FGF-21 and DCN in bovine muscle cells: Effects of age and differentiation. PLoS ONE 19(7): e0299975. https://doi.org/10.1371/journal.pone.0299975
