Pyrene Fate Affected by Humic Acid Amendment in Soil Slurry Systems

Authors

Y. Liang
D. Sorensen
Ronald C. Sims, Utah State UniversityFollow
Joan McLean

Document Type

Article

Journal/Book Title

Journal of Biological Engineering

Publication Date

2008

Volume

2

Abstract

Background

Humic acid (HA) has been found to affect the solubility, mineralization, and bound residue formation of polycyclic aromatic hydrocarbons (PAHs). However, most of the studies on the interaction between HA and PAH concentrated on one or two of the three phases. Few studies have provided a simple protocol to demonstrate the overall effects of HA on PAH distribution in soil systems for all three phases.

Methods

In this study, three doses of standard Elliott soil HA (ESHA), 15, 187.5, and 1,875 μg ESHA/g soil slurry, were amended to soil slurry systems. ¹⁴C-pyrene was added to the systems along with non-radiolabeled pyrene; ¹⁴C and ¹⁴CO₂ were monitored for each system for a period of 120 days.

Results

The highest amendment dose significantly increased the ¹⁴C fraction in the aqueous phase within 24 h, but not after that time. Pyrene mineralization was significantly inhibited by the highest dose over the 120-day study. While organic solvent extractable ¹⁴C decreased with time in all systems, non-extractable or bound ¹⁴C was significantly enhanced with the highest dose of ESHA addition.

Conclusion

Amendment of the highest dose of ESHA to pyrene contaminated soil was observed to have two major functions. The first was to mitigate CO₂ production significantly by reducing ¹⁴CO₂ from ¹⁴C pyrene mineralization. The second was to significantly increase stable bound ¹⁴C formation, which may serve as a remediation end point. Overall, this study demonstrated a practical approach for decontamination of PAH contaminated soil. This approach may be applicable to other organic contaminated environments where active bioremediation is taking place.

Comments

Originally published by BioMed Central.

Recommended Citation

Liang, Y.; Sorensen, D.; Sims, Ronald C.; and McLean, Joan, "Pyrene Fate Affected by Humic Acid Amendment in Soil Slurry Systems" (2008). Biological Engineering Faculty Publications. Paper 1.
https://digitalcommons.usu.edu/bioeng_facpub/1