Document Type
Article
Journal/Book Title
BMC Research Notes
Publication Date
2016
Publisher
BioMed Central
Volume
9
Issue
338
Abstract
Background
One of the potential limitations of large scale aerobic Escherichia coli fermentation is the need for increased dissolved oxygen for culture growth and bioproduct generation. As culture density increases the poor solubility of oxygen in water becomes one of the limiting factors for cell growth and product formation. A potential solution is to use a microbubble dispersion (MBD) generating device to reduce the diameter and increase the surface area of sparged bubbles in the fermentor. In this study, a recombinantE. coli strain was used to produce polyhydroxybutyrate (PHB) under conventional and MBD aerobic fermentation conditions.
Results
In conventional fermentation operating at 350 rpm and 0.8 vvm air flow rate, an OD600 of 6.21 and PHB yield of 23 % (dry cell basis) was achieved. MBD fermentation with similar bioreactor operating parameters produced an OD600 of 8.17 and PHB yield of 43 % PHB, which was nearly double that of the conventional fermentation.
Conclusions
This study demonstrated that using a MBD generator can increase oxygen mass transfer into the aqueous phase, increasing E. coli growth and bioproduct generation.
First Page
1
Last Page
7
Recommended Citation
Innan, Kadriye; Sal, Fulya Ay; Rahman, Asif; Putman, Ryan J.; Agblevor, Foster A.; and Miller, Charles D., "Microbubble assisted polyhydroxybutyrate production in Escherichia coli" (2016). Biological Engineering Faculty Publications. Paper 145.
https://digitalcommons.usu.edu/bioeng_facpub/145
