Document Type

Article

Author ORCID Identifier

Jaden Storrer https://orcid.org/0009-0008-7228-9276

Journal/Book Title/Conference

Bioresources and Bioproducts

Volume

1

Issue

2

Publisher

MDPI AG

Publication Date

11-1-2025

Journal Article Version

Version of Record

First Page

1

Last Page

18

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Abstract

Methane is a potent greenhouse gas that requires its emissions to be mitigated. A significant source for methane emissions is in the form of the biogas that is produced from anaerobic digestion in wastewater reclamation and landfill facilities. Biogas has a high valorization potential in the form of its bioconversion into ectoines, an active ingredient in skin care products, by halotolerant alkaliphilic methanotrophs. Cultures of Methylotuvimicrobium alcaliphilum 20Z were grown in bench scale stirred-tank reactors to determine factors to improve methane uptake and removal. Tangential flow filtration was also implemented for a bio-milking method to recover ectoine from culture media. Methane uptake and reactor productivity increased, with a temperature of 28 ◦C compared with 21 ◦C. Decreasing the methane gas bubble diameter by decreasing the sparger pore size from 1 mm to 0.5 µm significantly improved methane removal and reactor productivity by increasing mass transfer. Premixing methane and air before sparging into the reactor saw a higher removal of methane, while sparging methane and air separately created an increase in reactor productivity. Maximum methane removal efficiency was observed to be 70.56% ± 0.54 which translated to a CH4-EC of 93.82 ± 3.36 g CH4 m−3 h −1 . Maximum ectoine yields was observed to be 0.579 mg ectoine L−1 h −1 .

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