Date of Award:
5-1-1975
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Department name when degree awarded
Bacteriology and Public Health
Committee Chair(s)
Larre N. Egbert
Committee
Larre N. Egbert
Committee
Rex S. Spendlove
Committee
Paul B. Carter
Abstract
The purpose of this study was to determine if Bacillus kaustophilus L-asparaginase is capable of limiting the growth rates of Walker rat carcinosarcoma 256 (LLC-WRC-256) and Jensen sarcoma cells as compared to Escherichia coli EC-II L-asparaginase. Cell cultures of MDBK and MDCK as controls and LLC-WRC-256 and Jensen sarcoma cells as test cultures which are sensitive to EC-II were grown in standard growth media containing either B. kaustophilus L-asparaginase, EC-II or no enzyme. Cultures of each cell type in each medium were terminated on a fixed schedule and assayed for total protein and total DNA content. The changes in protein and DNA content were compared as a function of time to determine the increase in cell populations. The findings of this study indicated that B. kaustophilus L-asparaginase did not inhibit the in vitro growth of EC-II sensitive cells.
Recommended Citation
Coulter, Dean Marshall, "Effect of Bacillus kaustophilus L-Asparaginase on the Growth of Mammalian Cells of Tumor and Nontumor Origin" (1975). Biology. 408.
https://digitalcommons.usu.edu/etd_biology/408
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