Date of Award:
5-1-2004
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Committee Chair(s)
Daryll B. DeWald
Committee
Daryll B. DeWald
Committee
Gregory J. Podgorski
Committee
Noelle E. Cockett
Abstract
During dehydration and cold stress, Arabidopsis thaliana (A. thaliana) plant cells mobilize calcium into their cytosol to elicit changes in gene expression that enable them to tolerate stress. It has been unclear how dehydration-stressed plant cells receive and transmit the signal that results in promoting increases in intracellular calcium concentration. Previously, phosphatidylinositol 4,5-biphosphate (PtdIns(4,5)P2) and inositol 1,4,5 triphosphate (Ins(1,4,5)P3) have been shown to accumulate and cause calcium mobilization in osmotically stressed A. thaliana. However, it was unknown whether the same occurred in plants exposed to other abiotic stresses. Data presented in this thesis show that dehydration-stressed A. thaliana also accumulate PtdIns(4,5)P2 and Ins(1,4,5)P3, potentially leading to calcium mobilization. Although 1 h cold-stressed A. thaliana accumulate PtdIns(4,5)P2 in both shoot and root tissues with a large accumulation detected in root tissues, there was a reduction in the simultaneously measured Ins(1,4,5)P3 levels. In summary, these data show that PtdIns(4,5)P2 accumulates in dehydration and cold-stressed A. thaliana while Ins(1,4,5)P3 accumulates in dehydration-stressed plants. This suggests that the phosphoinositide signaling pathway is activated during dehydration and cold stress resulting in calcium mobilization.
Recommended Citation
Thompson, James E., "Analysis of Phosphatidylinositol 4,5-Biphosphate and Inositol 1,4,5-Triphosphate in Dehydration and Cold-Stressed Arabidopsis thaliana" (2004). Biology. 697.
https://digitalcommons.usu.edu/etd_biology/697
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