Current Issue: Volume 1996, Number 10 (1996) The Science of Biological Specimen Preparation for Microscopy

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Comparative Scanning, Transmission and Atomic Force Microscopy of the Microtubular Cytoskeleton in Fenestrated Liver Endothelial Cells
Filip Braet, Ronald De Zanger, Wouter Kalle, Anton Raap, Hans Tanke, and Eddie Wisse

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Specimen Preparation of the Human Cerebellar Cortex for Scanning Electron Microscopy Using a t-Butyl Alcohol Freeze-Drying Device
Teruyuki Hojo

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Neural Transplant Staining with DiI and Vital Imaging by 2-Photon Laser-Scanning Microscopy
Steve M. Potter, Jerome Pine, and Scott E. Fraser

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Multiple Labeling in Electron Microscopy: Its Application in Cardiovascular Research
M. M. H. Marijianowski, P. Teeling, K. P. Dingemans, and A. E. Becker

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Hydration-Scanning Tunneling Microscopy as a Reliable Method for Imaging Biological Specimens and Hydrophilic Insulators
M. Heim, R. Eschrich, A. Hillebrand, H. F. Knapp, G. Cevc, and R. Guckenberger

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Imaging Soft Materials with Scanning Tunneling Microscopy
J. T. Woodward IV and J. A. Zasadzinski

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Cytoskeleton Architecture of C6 Rat Glioma Cell Subclones Whole Mount Electron Microscopy and Immunogold Labeling
Wolfgang Bohn, Dörte Etzrodt, Roland Foisner, Gerhard Wiche, and Peter Traub

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Accessing Nuclear Structure for Field Emission, in Lens, Scanning Electron Microscopy (FEISEM)
T. D. Allen, G. R. Bennion, S. A. Rutherford, S. Reipert, A. Ramalho, E. Kiseleva, and M. W. Goldberg

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In Vitro Systems and Cultured Cells as Specimens for X-Ray Microanalysis
Godfried M. Roomans, Jarin Hongpaisan, Zhengzhu Jin, Ann-Christin Mörk, and Ailing Zhang

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Imaging Molecular Structure of Channels and Receptors with an Atomic Force Microscope
Ratneshwar Lal

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Atomic Force Microscopy of DNA, Nucleoproteins and Cellular Complexes: The Use of Functionalized Substrates
Yuri L. Lyubchenko, Robert E. Blankenship, Alexander A. Gall, S. M. Lindsay, Ottavio Thiemann, Larry Simpson, and Luda S. Shlyakhtenko

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X-Ray Microscopy: Preparations for Studies of Frozen Hydrated Specimens
A. Osanna, C. Jacobsen, A. Kalinovsky, J. Kirz, J. Maser, and S. Wang

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Preparation of Plasmid DNA in Transfection Complexes for Fluorescence and Electron Spectroscopic Imaging
Marek Malecki

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Problems in Preparation of Chromosomes for Scanning Electron Microscopy to Reveal Morphology and to Permit Immunocytochemistry of Sensitive Antigens
A. T. Sumner

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Generation of High Efficiency ssDNA Hybridization Probes by Linear Polymerase Chain Reaction (LPCR)
Gregory W. Konat

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Pre-Embedding Staining of Single Muscle Fibers for Light and Electron Microscopy Studies of Subcellular Organization
Evelyn Ralston and Thorkil Ploug

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Advanced Instrumentation and Methodology Related to Cryoultramicrotomy: A Review
H. Sitte

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Labeling with Nanogold and Undecagold: Techniques and Results
James F. Hainfeld

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Covalent Labeling of Proteins with Fluorescent Compounds for Imaging Applications
Darl R. Swartz

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Emerging Applications of Fluorescence Spectroscopy to Cellular Imaging: Lifetime Imaging, Metal-Ligand Probes, Multi-Photon Excitation and Light Quenching
Joseph R. Lakowicz

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Nucleic Acid Detection by In Situ Molecular Immunogold Labeling Procedures
Marc Thiry

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Freeze-Dried Human Leukocytes Stabilized with Uranyl Acetate During Low Temperature Embedding or with OsO4 Vapor After Embedding
L. Edelmann and A. Ruf

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Simultaneous Identification of a Specific Gene Protein Product and Transcript Using Combined Immunocytochemistry and In Situ Hybridization with Non-Radioactive Probes
Gwen V. Childs

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Video Rate Confocal Laser Scanning Reflection Microscopy in the Investigation of Normal and Neoplastic Living Cell Dynamics
Pavel Vesely and Alan Boyde

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Electro-Optical Imaging of F-Actin and Endoplasmic Reticulum in Living and Fixed Plant Cells
Nina Stromgren Allen and Marty N. Bennett

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Aspects of Cryofixation and Cryosectioning for the Observation of Bulk Biological Samples in the Hydrated State by Cryoelectron Microscopy
K. Richter

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Preparation of Samples for Polymerase Chain Reaction In Situ
Gerard J. Nuovo

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Microscopic Analysis of DNA and DNA-Protein Assembly by Transmission Electron Microscopy, Scanning Tunneling Microscopy and Scanning Force Microscopy
T. Müller-Reichhert and H. Gross

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In Situ Hybridization, In Situ Transcription, and In Situ Polymerase Chain Reaction
L. E. De Bault and J. Gu

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Correlated Confocal and Intermediate Voltage Electron Microscopy Imaging of the Same Cells Using Sequential Fluorescence Labeling, Fixation, and Critical Point Dehydration
Lee D. Peachey, Harunori Ishikawa, and Tohru Murakami

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Immunocytochemistry by Electron Spectroscopic Imaging Using Well Defined Boronated Monovalent Antibody Fragments
M. M. Kessels, B. Qualmann, and W. D. Sierralta