Current Issue: Volume 1996, Number 10 (1996) The Science of Biological Specimen Preparation for Microscopy

PDF

Preparation of Plasmid DNA in Transfection Complexes for Fluorescence and Electron Spectroscopic Imaging
Marek Malecki

PDF

Simultaneous Identification of a Specific Gene Protein Product and Transcript Using Combined Immunocytochemistry and In Situ Hybridization with Non-Radioactive Probes
Gwen V. Childs

PDF

In Situ Hybridization, In Situ Transcription, and In Situ Polymerase Chain Reaction
L. E. De Bault and J. Gu

PDF

Preparation of Samples for Polymerase Chain Reaction In Situ
Gerard J. Nuovo

PDF

Generation of High Efficiency ssDNA Hybridization Probes by Linear Polymerase Chain Reaction (LPCR)
Gregory W. Konat

PDF

Nucleic Acid Detection by In Situ Molecular Immunogold Labeling Procedures
Marc Thiry

PDF

Hydration-Scanning Tunneling Microscopy as a Reliable Method for Imaging Biological Specimens and Hydrophilic Insulators
M. Heim, R. Eschrich, A. Hillebrand, H. F. Knapp, G. Cevc, and R. Guckenberger

PDF

Imaging Molecular Structure of Channels and Receptors with an Atomic Force Microscope
Ratneshwar Lal

PDF

Atomic Force Microscopy of DNA, Nucleoproteins and Cellular Complexes: The Use of Functionalized Substrates
Yuri L. Lyubchenko, Robert E. Blankenship, Alexander A. Gall, S. M. Lindsay, Ottavio Thiemann, Larry Simpson, and Luda S. Shlyakhtenko

PDF

Microscopic Analysis of DNA and DNA-Protein Assembly by Transmission Electron Microscopy, Scanning Tunneling Microscopy and Scanning Force Microscopy
T. Müller-Reichhert and H. Gross

PDF

Imaging Soft Materials with Scanning Tunneling Microscopy
J. T. Woodward IV and J. A. Zasadzinski

PDF

Accessing Nuclear Structure for Field Emission, in Lens, Scanning Electron Microscopy (FEISEM)
T. D. Allen, G. R. Bennion, S. A. Rutherford, S. Reipert, A. Ramalho, E. Kiseleva, and M. W. Goldberg

PDF

Problems in Preparation of Chromosomes for Scanning Electron Microscopy to Reveal Morphology and to Permit Immunocytochemistry of Sensitive Antigens
A. T. Sumner

PDF

Electro-Optical Imaging of F-Actin and Endoplasmic Reticulum in Living and Fixed Plant Cells
Nina Stromgren Allen and Marty N. Bennett

PDF

Neural Transplant Staining with DiI and Vital Imaging by 2-Photon Laser-Scanning Microscopy
Steve M. Potter, Jerome Pine, and Scott E. Fraser

PDF

Video Rate Confocal Laser Scanning Reflection Microscopy in the Investigation of Normal and Neoplastic Living Cell Dynamics
Pavel Vesely and Alan Boyde

PDF

Emerging Applications of Fluorescence Spectroscopy to Cellular Imaging: Lifetime Imaging, Metal-Ligand Probes, Multi-Photon Excitation and Light Quenching
Joseph R. Lakowicz

PDF

Comparative Scanning, Transmission and Atomic Force Microscopy of the Microtubular Cytoskeleton in Fenestrated Liver Endothelial Cells
Filip Braet, Ronald De Zanger, Wouter Kalle, Anton Raap, Hans Tanke, and Eddie Wisse

PDF

Correlated Confocal and Intermediate Voltage Electron Microscopy Imaging of the Same Cells Using Sequential Fluorescence Labeling, Fixation, and Critical Point Dehydration
Lee D. Peachey, Harunori Ishikawa, and Tohru Murakami

PDF

Pre-Embedding Staining of Single Muscle Fibers for Light and Electron Microscopy Studies of Subcellular Organization
Evelyn Ralston and Thorkil Ploug

PDF

Multiple Labeling in Electron Microscopy: Its Application in Cardiovascular Research
M. M. H. Marijianowski, P. Teeling, K. P. Dingemans, and A. E. Becker

PDF

Covalent Labeling of Proteins with Fluorescent Compounds for Imaging Applications
Darl R. Swartz

PDF

Cytoskeleton Architecture of C6 Rat Glioma Cell Subclones Whole Mount Electron Microscopy and Immunogold Labeling
Wolfgang Bohn, Dörte Etzrodt, Roland Foisner, Gerhard Wiche, and Peter Traub

PDF

Freeze-Dried Human Leukocytes Stabilized with Uranyl Acetate During Low Temperature Embedding or with OsO4 Vapor After Embedding
L. Edelmann and A. Ruf

PDF

Labeling with Nanogold and Undecagold: Techniques and Results
James F. Hainfeld

PDF

Immunocytochemistry by Electron Spectroscopic Imaging Using Well Defined Boronated Monovalent Antibody Fragments
M. M. Kessels, B. Qualmann, and W. D. Sierralta

PDF

Specimen Preparation of the Human Cerebellar Cortex for Scanning Electron Microscopy Using a t-Butyl Alcohol Freeze-Drying Device
Teruyuki Hojo

PDF

X-Ray Microscopy: Preparations for Studies of Frozen Hydrated Specimens
A. Osanna, C. Jacobsen, A. Kalinovsky, J. Kirz, J. Maser, and S. Wang

PDF

In Vitro Systems and Cultured Cells as Specimens for X-Ray Microanalysis
Godfried M. Roomans, Jarin Hongpaisan, Zhengzhu Jin, Ann-Christin Mörk, and Ailing Zhang

PDF

Aspects of Cryofixation and Cryosectioning for the Observation of Bulk Biological Samples in the Hydrated State by Cryoelectron Microscopy
K. Richter

PDF

Advanced Instrumentation and Methodology Related to Cryoultramicrotomy: A Review
H. Sitte