Class
Article
College
College of Engineering
Department
Biological Engineering Department
Faculty Mentor
Elizabeth Vargis
Presentation Type
Poster Presentation
Abstract
Forming blood vessels is a common obstacle that is faced when attempting to develop artificial organs. One possible method of overcoming this obstacle is by using a 3D printed material as a scaffold for blood vessel growth. In previous studies, it has been shown that Poly-lactic acid (PLA) is an ideal material for use as a scaffold in tissue engineering applications (Kudryavtseva et al., 2017). The following proof of concept will evaluate the ability of PLA to support cell growth on its surface. Specifically, this project aims to determine its usefulness as a scaffold in the culture of Adult Retinal Pigment Epithelium cells (ARPE-19) in the shape of a blood vessel. This was done by 3D printing scaffolds out of PLA, in both a flat sheet and various shapes to promote tubular cell formation. Cells were then cultured on the scaffolds and incubated for enough time to reach confluency. A viability assay was then conducted to determine the effectiveness of PLA at supporting cell growth. It was found that PLA could support the growth of ARPE-19 cells, but it will be necessary to perform further testing to be able to determine how the different shapes affect the the growth of the cells.Presentation Time: Wednesday, 9-10 a.m.
Location
Logan, UT
Start Date
4-11-2021 12:00 AM
Included in
Growth of ARPE Cells on a 3D Printed PLA Scaffold
Logan, UT
Forming blood vessels is a common obstacle that is faced when attempting to develop artificial organs. One possible method of overcoming this obstacle is by using a 3D printed material as a scaffold for blood vessel growth. In previous studies, it has been shown that Poly-lactic acid (PLA) is an ideal material for use as a scaffold in tissue engineering applications (Kudryavtseva et al., 2017). The following proof of concept will evaluate the ability of PLA to support cell growth on its surface. Specifically, this project aims to determine its usefulness as a scaffold in the culture of Adult Retinal Pigment Epithelium cells (ARPE-19) in the shape of a blood vessel. This was done by 3D printing scaffolds out of PLA, in both a flat sheet and various shapes to promote tubular cell formation. Cells were then cultured on the scaffolds and incubated for enough time to reach confluency. A viability assay was then conducted to determine the effectiveness of PLA at supporting cell growth. It was found that PLA could support the growth of ARPE-19 cells, but it will be necessary to perform further testing to be able to determine how the different shapes affect the the growth of the cells.Presentation Time: Wednesday, 9-10 a.m.