Class
Article
College
College of Engineering
Department
Biological Engineering Department
Faculty Mentor
Elizabeth Vargis
Presentation Type
Poster Presentation
Abstract
Native and transgenic silkworm silk have been shown to be capable of supporting skeletal muscle cell growth with the use of a three-dimensional acrylic cell-culture chassis. Additionally, this silk has been shown to be preferable to the standard two-dimensional polystyrene cell culture surface in its ability to simulate native tissue 1. The protein threads produced from hagfish slime have also been shown to have potential applications in three-dimensional cell culture 2. To compare the capacity for three-dimensional cell growth and simulation of native tissue to native and transgenic silkworm silk, hagfish protein threads were synthesized and woven around an acrylic chassis and seeded with C2C12 myoblasts. The ability of the protein threads to support three-dimensional cell growth, uni-directional myofiber growth, and independent fiber anchorage was evaluated with brightfield imaging, as well as fluorescent and immunocytochemical staining. Compared to silkworm silk, hagfish protein thread has improved myofiber alignment, increased cell growth, and greater cell anchorage when used in cell culture. Presentation Time: Thursday, 12-1 p.m.
Location
Logan, UT
Start Date
4-11-2021 12:00 AM
Included in
Hagfish Protein Threads as Three Dimensional In Vitro Scaffolds for Skeletal Muscular Tissue
Logan, UT
Native and transgenic silkworm silk have been shown to be capable of supporting skeletal muscle cell growth with the use of a three-dimensional acrylic cell-culture chassis. Additionally, this silk has been shown to be preferable to the standard two-dimensional polystyrene cell culture surface in its ability to simulate native tissue 1. The protein threads produced from hagfish slime have also been shown to have potential applications in three-dimensional cell culture 2. To compare the capacity for three-dimensional cell growth and simulation of native tissue to native and transgenic silkworm silk, hagfish protein threads were synthesized and woven around an acrylic chassis and seeded with C2C12 myoblasts. The ability of the protein threads to support three-dimensional cell growth, uni-directional myofiber growth, and independent fiber anchorage was evaluated with brightfield imaging, as well as fluorescent and immunocytochemical staining. Compared to silkworm silk, hagfish protein thread has improved myofiber alignment, increased cell growth, and greater cell anchorage when used in cell culture. Presentation Time: Thursday, 12-1 p.m.