Class

Article

College

College of Agriculture and Applied Sciences

Department

Animal, Dairy, and Veterinary Sciences Department

Faculty Mentor

Ying Liu

Presentation Type

Poster Presentation

Abstract

  • The metabolic pathways that oocytes and embryos take during early development have an important effect on epigenetic events. This leads to the question of whether different cultures affect epigenetics.
  • There are some concerns that serum-containing mediums may be correlated with poor health outcomes.
  • Acetylation of lysine 9 on histone 3 is associated with chromatin configurations. The intensity of H3K9ac is much lower in cloned embryos (somatic cell nuclear transfer (SCNT)) than what is seen in normal embryos
  • This study was aiming to compare IVF and SCNT embryos cultured in a medium containing serum and a medium without serum. The medium containing serums is a homemade synthetic oviduct fluid (SOF) with FBS medium. The medium without serum is BO-IVC and BO-IVM.
  • This study was also focused on comparing the acetylation of lysine 9 on histone 3 epigenetic marker

Location

Logan, UT

Start Date

4-10-2024 10:30 AM

End Date

4-10-2024 11:20 AM

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Apr 10th, 10:30 AM Apr 10th, 11:20 AM

Effect of Medium on Epigenetics in Fertilized and Cloned Embryos

Logan, UT

  • The metabolic pathways that oocytes and embryos take during early development have an important effect on epigenetic events. This leads to the question of whether different cultures affect epigenetics.
  • There are some concerns that serum-containing mediums may be correlated with poor health outcomes.
  • Acetylation of lysine 9 on histone 3 is associated with chromatin configurations. The intensity of H3K9ac is much lower in cloned embryos (somatic cell nuclear transfer (SCNT)) than what is seen in normal embryos
  • This study was aiming to compare IVF and SCNT embryos cultured in a medium containing serum and a medium without serum. The medium containing serums is a homemade synthetic oviduct fluid (SOF) with FBS medium. The medium without serum is BO-IVC and BO-IVM.
  • This study was also focused on comparing the acetylation of lysine 9 on histone 3 epigenetic marker