Advancing Space Flight Medical Care through On-demand Protein Therapeutic Production Capabilities

Cell-free protein synthesis (CFPS) is an in vitro protein synthesis system that provides a uniquely adaptable platform for protein production. In recent years, lyophilization of CFPS have expanded the capabilities of this system by improving reagent stability outside of cold-chain storage. This project sought to utilize lyophilized CFPS reagents to develop a convenient, shelf-stable, on-demand therapeutic protein production platform. The therapeutic protein production potential of this novel platform was demonstrated by the successful expression of an FDA approved therapeutic protein. With the advancement of shelf-stable, on-demand protein therapeutic production platforms comes the potential to provide point-of-care medical treatment for space-flight crews during long duration missions.